Continued">Continued">Continued">Immunostaining (IHC) & fluorescent in-situ hybridization (FISH) – Children Cancer Hospital Egypt 57357

Immunostaining (IHC) & fluorescent in-situ hybridization (FISH)

Immunostaining tests are rigorously validated before implementation for clinical diagnosis and is focused on diagnostic, predictive and prognostic protein/markers (antigens).

  • VENTANA autostainers (BenchMark ULTRA).
  • Over 100 immunostaining diagnostic, prognostic & predictive tests e.g. HER2/neu, C-myc, C-Kit, Ki-67 … etc.

FISH is a unique service based on staff expertise, instrumentation & a comprehensive marker list of tests.

  • Available FISH tests for clinical diagnosis:
    • CMYC amplification.
    • NMYC amplification.
    • FOXO rearrangement.
    • EWSR rearrangement.
    • Synovial sarcoma rearrangement.
  • A much more is always added, keep tuned…
Available Immunostaining Panels
Hematolymphoid
CD20CD43
CD163CD19
CYCLIND-1CD3
CD138CD4
CD8CD13
CD30CD33
CD15 CD23
CD1A CD7
CD56 cmyc
CD61 ALK
CD99 CD31
BCL6 CD5
BCL2 CD10
CD79a EBV
TdT PAX5
CD21 MPO (MYELOPEROXIDASE)
CD3 CD207 (Langerin)
CD34 GLYCOPHORIN
CD68CD45LCA
MUM-1
Soft tissue & Bone
BCORCalponin
Factor VIIIS100
ERGDesmin
DOG-1ETV6
SDHBTFE3
h-caldesmonNUT-1
SOX10STAT-6
ETV4MYOGNIN
B-CateninWTI
C-KIT (CD117)IH SMA
EGFRFACTOR XIIIa
NESTININI
TLE-1FLI-1
NKX-2ALK
CD99EMA
Carcinoma work-up
CKCalcitonin
EMAD2-40
CK7GLYPICAN3
CK20CK19
CDX-2HBME-1
SAT-B2HMW CK
TTF-1HEPPAR-1
SynaptophysinAFP
ChromograninP63
InhibinCALRETININ
THYROGLOBULINHMB45
MMR-4 markers
CEA
Breast Cancer Hormonal Panel
ERHER2-NEU
PRKi-67
CNS Panel
OLig2Inhibin
EMA CONCEMA
p53Synaptophysin
YAP-1CK
LIN28B-catenin
GAB-1CD117
GFAPINI
Targetable
PDL-1

FISH Panels
Hematolymphoid Panel
IGH/MYC FUSION (FISH)BCL6 FUSION (FISH)
Aggressive B-cell Lymphoma FUSION pnel (FISH)BCL lymphoma FUSION Panel (FISH)
BCL2 FUSION (FISH)
Soft tissue/pediatric Panel
Ewing Sarcoma FUSION (FISH)Synovial Sarcoma FUSION (FISH)
FOXO FUSION (FISH)MYCN amplification (FISH)
CNS Panel
EGFR CNV (FISH)C19MC /19p13 (FISH)
Other FISH TESTs
NTRK FUSION (FISH)EGFR (CNV) FISH

N.B. Targetable, CNS & hematolymphoid FISH panels will be available within 1 month