Surgical Pathology Internship-2023
Course overview
During this internship, participants will understand what is peculiar for surgical pathology; types of samples received by any surgical pathology lab, different facilities in pathology lab (surgical pathology, cytopathology, molecular pathology, digital pathology, immunostaining, FISH and special stains) and how to deal with laboratory information system (LIS). they will get to know more about sops of lab procedures, quality, infection control and ethics involved in lab practice.
Learning Objectives
By the end of this training participants will be able to:
Surgical pathology training objectives
- Know Basic lab skills from sample receiving, grossing, fixation and processing.
- Understand principles of H&E slide staining.
- Understand advancements and updates of technical aspects in pathology Lab facilities.
- Know the Workflow with 57357 CCHE (both manual and automated).
- Know the Skills of paraffin block handling and cutting in different tissue samples.
Immunostaining training objectives
- Understand immunostaining mechanism.
- Understand the difference between the immunostaining and other stains.
- Make Slide handling and preparation for immunostaining.
- Understand different immunostaining protocols.
- know the Skills of preparing immunohistochemistry autostainers .
- do Immunostaining troubleshoot.
- Understand the importance of immunostained slides evaluation before diagnosis
Florescent in situ Hybridization (FISH) training objectives:
- Understand FISH mechanism.
- Understand the difference between the FISH and other ancillary techniques.
- Slide handling and preparation for FISH.
- Understand different FISH protocols.
- Develop skills of preparing FISH reagents.
- Recognize fish troubleshooting.
- Understand the importance of FISH slides evaluation before diagnosis.
Cytology training objectives:
- Know the types of cytology samples.
- know the differences between pathology and cytology in preparation & staining.
- develop the skills of cytology samples handling and preparation.
- develop the skills of cell block preparation.
- Know the importance of preparing cell block from cytology sample.
- Know the differences between conventional cytology and liquid based cytology preparation.
- Apply immunostaining and molecular testing in different cytology samples.
- Recognize Troubleshooting in cytology techniques.
Digital pathology training objectives:
- Know what digital pathology is.
- know the benefits of digital pathology in diagnosis and research.
- Develop the Skills of scanning protocols optimization.
- Understand different scanning protocols.
- Develop the skills of slide preparation before scanning.
- Develop the skills of slide evaluation after scanning.
Molecular pathology training objectives:
- Understand the basics of molecular oncology.
- Understand of the basic principles of nucleic acid purification.
- Understanding the difference between the somatic and germline mutations.
- Nucleic acid isolation and manipulations from FFPE.
- Understand different real time PCR applications in molecular pathology.
- Design and run a Real-Time PCR experiment.
- Troubleshoot real time PCR
Duration
2 weeks
Mode of delivery
onsite
Program Schedule
| Day | From | To | Topic | Type |
| Sunday | 10:00 | 16:00 | Introduction, Sample accession Fixation | Didactic & Practical |
| Monday | 10:00 | 16:00 | Grossing room Decalcification of bony samples | Practical |
| Tuesday | 10:00 | 16:00 | Tissue processing: Manual tissue processing Automated tissue processing | Didactic & Practical |
| Wednesday | 10:00 | 16:00 | Tissue embedding Cutting blocks (Microtome) | Practical |
| Thursday | 10:00 | 16:00 | Staining Principles; H&E staining, Special stains and frozen Section | Didactic & Practical |
| Sunday | 10:00 | 16:00 | Cytology: Types of cytology samples, Sample preparation Fixation, Types of cytology staining, Cell block preparation, Liquid based cytology. | Didactic and Practical |
| Monday | 10:00 | 16:00 | Immunostaining (IHC) -Introduction, Slide preparation, Quality control, Machine preparation, Steps of automated staining. Florescent in situ Hybridization(FISH) Introduction, Slide preparation, Quality control, Reagent preparation, Hybridization of FISH probs. | Didactic and Practical |
| Tuesday | 10:00 | 16:00 | Digital pathology, Introduction, Slide preparation, Scanner loading & operation, Protocol optimization, Quality control, Case assignment . | Practical |
| Wednesday | 10:00 | 16:00 | Molecular sample preparation: Nucleic acids isolation, Purification from FFPE block,RNA & DNA quality control. | Didactic and Practical |
| Thursday | 10:00 | 16:00 | Molecular Real Time PCR Chemistries Applications. | Didactic and Practical |
Available Dates
| Waves | Available dates |
| Wave 1 | From 02/07/2023 – 13/07/2023 |
| Wave 2 | From 16/07/2023 – 27/07/2023 |
| Wave 3 | From 30/07/2023 – 10/08/2023 |
| Wave 4 | From 13/08/2023 – 24/08/2023 |
| Wave 5 | From 27/08/2023 – 07/09/2023 |
| Wave 6 | From 10/09/2023 – 21/09/2023 |
| Wave 7 | From 24/09/2023 – 05/10/2023 |
Target Audience
Science Faculty of Sciences, Biotechnology, Agriculture