During this internship, participants will understand what is peculiar for surgical pathology; types of samples received by any surgical pathology lab, different facilities in pathology lab (surgical pathology, cytopathology, molecular pathology, digital pathology, immunostaining, FISH and special stains) and how to deal with laboratory information system (LIS). they will get to know more about sops of lab procedures, quality, infection control and ethics involved in lab practice.

By the end of this training participants will be able to:

Surgical pathology training objectives

  • Know Basic lab skills from sample receiving, grossing, fixation and processing.
  • Understand principles of H&E slide staining.
  • Understand advancements and updates of technical aspects in pathology Lab facilities.
  • Know the Workflow with 57357 CCHE (both manual and automated).
  •  Know the Skills of paraffin block handling and cutting in different tissue samples.

Immunostaining training objectives

  • Understand immunostaining mechanism.
  • Understand the difference between the immunostaining and other stains.
  • Make Slide handling and preparation for immunostaining.
  • Understand different immunostaining protocols.
  • know the Skills of preparing immunohistochemistry autostainers .
  • do Immunostaining troubleshoot.
  • Understand the importance of immunostained slides evaluation before diagnosis

Florescent in situ Hybridization (FISH) training objectives:

  • Understand FISH mechanism.
  • Understand the difference between the FISH and other ancillary techniques.
  • Slide handling and preparation for FISH.
  • Understand different FISH protocols.
  • Develop skills of preparing FISH reagents.
  • Recognize fish troubleshooting.
  • Understand the importance of FISH slides evaluation before diagnosis.

Cytology training objectives:

  • Know the types of cytology samples.
  •  know the differences between pathology and cytology in preparation & staining.
  • develop the skills of cytology samples handling and preparation.
  • develop the skills of cell block preparation.
  • Know the importance of preparing cell block from cytology sample.
  • Know the differences between conventional cytology and liquid based cytology preparation.
  •  Apply immunostaining and molecular testing in different cytology samples.
  • Recognize Troubleshooting in cytology techniques.

Digital pathology training objectives:

  • Know what digital pathology is.
  • know the benefits of digital pathology in diagnosis and research.
  • Develop the Skills of scanning protocols optimization.
  • Understand different scanning protocols.
  • Develop the skills of slide preparation before scanning.
  • Develop the skills of slide evaluation after scanning.

Molecular pathology training objectives:

  • Understand the basics of molecular oncology.
  • Understand of the basic principles of nucleic acid purification.
  • Understanding the difference between the somatic and germline mutations.
  • Nucleic acid isolation and manipulations from FFPE.
  • Understand different real time PCR applications in molecular pathology.
  •  Design and run a Real-Time PCR experiment.
  • Troubleshoot real time PCR

2 weeks


Sunday10:0016:00Introduction, Sample accession
Didactic & Practical
Monday10:0016:00Grossing room
Decalcification of bony samples
Tuesday10:0016:00Tissue processing:
Manual tissue processing
Automated tissue processing
Didactic & Practical
Wednesday10:0016:00Tissue embedding
Cutting blocks (Microtome)
Thursday10:0016:00Staining Principles; H&E staining, Special stains and frozen SectionDidactic & Practical
Sunday10:0016:00Cytology: Types of cytology samples, Sample preparation Fixation, Types of cytology staining, Cell block preparation, Liquid based cytology.Didactic and Practical 
Monday10:0016:00Immunostaining (IHC)
-Introduction, Slide preparation, Quality control, Machine preparation, Steps of automated staining.
Florescent in situ Hybridization(FISH)
Introduction, Slide preparation, Quality control, Reagent preparation, Hybridization of FISH probs.
Didactic and Practical
Tuesday10:0016:00Digital pathology, Introduction, Slide preparation, Scanner loading & operation, Protocol optimization, Quality control, Case assignment .Practical 
Wednesday10:0016:00Molecular sample preparation: Nucleic acids isolation, Purification from FFPE block,RNA & DNA quality control.Didactic and Practical
Thursday10:0016:00Molecular Real Time PCR
Didactic and Practical
WavesAvailable dates
Wave 1From 02/07/2023 – 13/07/2023
Wave 2From 16/07/2023 – 27/07/2023
Wave 3From 30/07/2023 – 10/08/2023
Wave 4From 13/08/2023 – 24/08/2023
Wave 5From 27/08/2023 – 07/09/2023  
Wave 6From 10/09/2023 – 21/09/2023  
Wave 7From 24/09/2023 – 05/10/2023  

Science Faculty of Sciences, Biotechnology, Agriculture

The fees of the course will be directed towards the treatment of our children.

Course Fees and Registration